This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, ... Zobraziť viac
RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, ... Zobraziť viac
An indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo... Zobraziť viac
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility... Zobraziť viac
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "next generation sequencing" methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place ... Zobraziť viac
Evidence-based medical practice stresses the examination of evidence from clinical research as the basis for clinical decision making, rather than relying on unsystematic clinical experience and pathophysiological rationale... Zobraziť viac
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology... Zobraziť viac
This volume covers the latest advancements and methods used to study DNA barcoding. The chapters in this book are organized into six parts: theory, DNA manipulation, specialized barcoding methods, analysis of DNA barcodes, completing a successful DNA barc Zobraziť viac
Data mining provides a set of new techniques to integrate, synthesize, and analyze tdata, uncovering the hidden patterns that exist within. Traditionally, techniques such as kernel learning methods, pattern recognition, and data mining, ... Zobraziť viac
This volume details state-of-the-art computational methods designed to manage, analyze, and generally leverage epigenomic and epitranscriptomic data. Chapters guide readers through fine-mapping and quantification of modifications, visual analytics,... Zobraziť viac
This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations... Zobraziť viac
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis... Zobraziť viac
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances... Zobraziť viac
The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing... Zobraziť viac
For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been... Zobraziť viac
This fifth edition was prompted by the need to provide up-to-date information to keep pace with the perpetual growth and improvement in the instrumentation and techniques employed in nuclear medicine since the last edition published in 2012. All... Zobraziť viac
